The contest uses a practical, verification-based definition: marine-derived ingredients are prohibited because current analytical tools cannot consistently differentiate non-extractive marine production from extractive sources. This rule is designed to keep compliance verifiable and the competition fair. If improved methods become available, F3 is open to discussing refinements. The F3 Team can be contacted at f3farmchallenge@gmail.com.

Non-extractive marine ingredients, such as land-based polychaetes grown in closed-loop systems, are not permitted under the current verification constraints. Even if a marine-derived ingredient is produced in a closed-loop or non-extractive way, the challenge cannot reliably distinguish it from wild-harvested marine ingredients using available analytical methods. For fairness and enforceability, the contest excludes marine-derived biological matter.

Additionally, fish or shrimp hydrolysates derived from trimmings or aquaculture waste are not permitted in the F3 Fish Farm Challenge. While F3 supports the broader use of circular and alternative ingredients in aquafeeds, these ingredients cannot be used in this contest because current analytical testing cannot reliably distinguish marine-derived hydrolysates, or other products, from prohibited marine animal ingredients. To ensure clear verification, all feeds used in the challenge must exclude these items.